戊四氮致痫大鼠情感反应、学习记忆功能及海马N-甲基-D-门冬氨酸受体mRNA表达的改变

CHANGE OF HIPPOCAMPAL NMDA RECEPTOR AND EMOTIONAL BEHAVIOR AND SPATIAL LEARNING AND MEMORY IN STATUS EPILEPTICUS RAT MODEL

目的:探讨实验性癫痫持续状态(SE)对大鼠认知功能的影响及N-甲基-D-门冬氨酸(NMDA)受体表达的变化。方法:戊四氮诱导大鼠SE,采用抬高迷宫和Morris水迷宫观察大鼠情感反应和学习记忆功能的改变。RT-PCR方法检测大鼠海马NMDA受体亚单位NR1 mRNA的表达。结果:SE组大鼠在抬高迷宫开放臂中逃避时间延长(P<0.01),进入次数增多(P<0.01);水迷宫中逃避潜伏期延长(P<0.01),搜寻策略变差(P<0.05),平台象限游泳时间百分比降低(P<0.01),穿越平台次数减少(P<0.01)。同时伴有海马NR1 mRNA表达下调(P<0.01)。结论:SE可使大鼠情感行为改变和学习记忆功能受损,NR1可能参与这一变化的病理生理过程。

Aim： SD rats were utilized for the purpose of the exploration of effects of status epilepticus（SE） on their emotional behavior, spatial learning and memory, and explorating its molecular mechanism. Methods： Forty maturity male SD rats, weighing（200 ＋ 20） g were divided randomly and equally into SE group（SG） and normal control group（NG）. The SG rats were induced by Pentylenete- trazole（PTZ） and the control animals received a saline（0.9% ）solution. The change of emotional behavior in two groups were tested in elevated plus maze. Furthermore, Morris water maze was applied to evaluate the effects by SE on spatial learning and memory in rats. At the same time, N-methyl-D-aspartate（NMDA） receptor NR1 subunit mRNA in the hippocampus was determined by reverse transcription polymerase chain reaction（RT-PCR）. Results： In elevated plus test, SE rats increased the times of visits as well as the time spent on the open arms of the elevated plus maze（ P〈 0.01 ）. In Morris water maze, the mean escape latency for the SE rats looking for hidden platform in the place navigation test prolonged（ P 〈 0.01 ）. The efficiency of their search strategy was poor（ P 〈 0.05）. The swimming time in platform region and the percentage of their swimming time decreased （P 〈 0.01 ）. The number of times they crossed the platform area decreased（ P 〈 0.01 ）. Meanwhile the expression of NR1 subunit mRNA in hippocampus was lower（P〈 0.01 ）. Conclusion： The experimental results showed that SE could result in the change of emotional behavior and damage of spatial learning and memory in rats. NR1 might be involved in the patho- and physiological process in causing these behavioral changes.