大豆异黄酮对围绝经期大鼠卵巢Bax mRNA表达和Ca^(2+)-ATP酶活性的影响

EFFCTS OF SOYISOFLAVONES ON THE EXPRESSION OF BAX mRNA AND Ca2+-ATPase ACTIVITY IN OVARIES OF PERIMENOPAUSE RATS

目的:观察大豆异黄酮(SI)对围绝经期大鼠卵巢Bax mRNA表达和Ca2+-ATP酶活性的影响。方法:采用自然老化法建立围绝经期大鼠动物模型。12月龄的初老雌性Wistar大鼠,分别给予低(50 mg/kg)、中(158 mg/kg)、高(500 mg/kg)剂量SI灌胃处理8周。采用RT-PCR检测卵巢Bax mRNA的表达;采用化学比色法检测卵巢Ca2+-ATP酶活性、血清丙二醛(MDA)含量和超氧化物岐化酶(SOD)活性。结果:SI处理可使初老大鼠卵巢BaxmRNA表达和血清MDA含量降低,卵巢Ca2+-ATP酶活性和血清SOD活性明显升高(P<0.05或P<0.01)。结论:大豆异黄酮下调衰老卵巢Bax mRNA表达,提高细胞Ca2+-ATP酶活性,可能是改善围绝经期卵巢功能的机制之一。

Aim： To investigate the effcts of soy isoflavones（SI） on the expression of Bax mRNA and Ca^2＋ -ATPase activity in ovaries of perimenopause rats. Methods： The animal model of perimenopause rats was established by unforced aging. 12 month-old praesenilis female Wistar rats were administered by intragastric（ig） with low（500 mg/kg）, middle（158 mg/kg） and high（500 mg/kg） does of SI for 8 weeks. The expression of Bax mRNA in ovaries were detected by RT-PCR. Ca^2＋ -ATPase activity in ovaries and MDA content and SOD activity in serum were detected by chemi-chromatometry. Results： Intervention of SI could significantly decrease the expression of Bax mRNA in ovaries and MDA content in serum, increase Ca^2＋ -ATPase activity in ovaries and SOD activity in serum of praesenilis rats（ P 〈 0.05 or P 〈 0.01 ）. Conclusion： Soyisoflavones could down-regulate the expression of Bax mRNA and increase Ca^2＋ -ATPase activity in aged ovaries. It is probably one of the mechanisms to improve the function of aged ovaries in perimenopause rats.