胃癌DAPK基因甲基化的初步研究

Studies on methylation of DAPK gene in primary gastric cancer

目的探讨死亡相关蛋白激酶(death-associate dprotein kinase,DAPK)基因甲基化与胃癌的关系。方法采用甲基化特异性PCR(methylation specific PCR,MSP)检测38例配对胃癌组织、癌旁正常组织和转移淋巴结中DAPK基因甲基化的情况。结果81.6%(31/38)的胃癌组织中存在异常甲基化,而相应的癌旁正常组织和转移淋巴结中该基因的甲基化率分别为42.1%(16/38)和70.2%(27/38)。癌组织和转移淋巴结中DAPK基因甲基化的发生率显著高于癌旁正常组织。癌旁正常组织中,该基因甲基化与年龄相关,P=0.020。但与肿瘤大体类型、分化程度及浸润深度等临床病理特征无关。结论DAPK基因异常甲基化是胃癌发生发展过程中的频发事件,通过检测胃黏膜组织及转移淋巴结中该基因的甲基化情况,可能会对胃癌的诊断及判断淋巴结的微转移提供一定的参考价值,并有望成为胃癌新的基因治疗靶点。

OBJECTIVE： To study the methylation status of DAPK gene in human gastric cancer. METHODS： Methylation status of DAPK gene in 38 gastric carcinoma specimens, corresponding non-neoplastic gastric mucosae and lymphonode was detected by methylationspecific PCR. RESULTS： Aberrant methylation was detected in 42.1% （16/38） of non-neoplastic gastric mucosac, 81.6% （31/38） of gastric carcinoma and 70.2% （27/38） of lymphonode. Methylation in the former was significantly less frequent than that in the others. Presence of DAPK methylation in non-neoplastic gastric mucosae was significantly correlated with old age. CONCLUSIONS： Aberrant methylation of DAPK gene is a common event in the development and progress of gastric cancer,it may provide useful information for the dignos is and assess ment of occult metastasis in lymphonode.