大肠癌组织中Bcl-2表达与临床病理参数相关性的研究

Study of expression of Bcl-2 in colorectal carcinoma and normal tissues

目的探讨抑制凋亡基因Bcl-2在大肠癌及癌旁组织中的表达及意义。方法应用免疫组化S-P法检测72例大肠癌组织和48例癌旁组织中Bcl-2的表达情况。结果Bcl-2在癌组织与癌旁组织的表达率分别是54.17%(39/72)和20.83%(10/48),二者差异有统计学意义,P<0.001。Bcl-2在高、中和低分化腺癌组织中表达分别是66.67%、47.06%和35.71%,统计学分析高、低分化腺癌之间差异有统计学意义,P=0.016。大肠癌组织Bcl-2表达与年龄、性别和瘤体大体分型等临床病理参数无关,P>0.05。在伴有淋巴结转移癌组织中Bcl-2表达率为34.78%,无淋巴结转移者为63.27%,统计学分析差异有统计学意义,P=0.024。DukeD期Bcl-2表达率显著低于A、B、C期,P<0.05。结论Bcl-2蛋白参与了大肠癌的发生,其表达高低与肿瘤细胞的病理分级、Duke临床分期和淋巴结转移密切相关。

OBJECTIVE： To investigate the relationship between the expression of Bcl-2 in the colorectal carcinoma and clinicopathologic features. METHODS： Sevenily-two specimens of carcinoma tissues and 48 specimens of transitional mucosas were examinated by S-P immunohistochemistry staining. Staining intensity in tumor cells was graded 0 to 3＋. RESULTS： The frequency of Bcl-2 staining in carcinoma tissues was 54.17% （39/72）, being significantly higher than that of in transitional mucosas 20.83% （10/48） （P〈0.001）. The positive rates in the high, moderate and low differentiated tissue were 66.67%（22/33）, 47.06%（8/17） and 35.71% （5/14）, respectively. A statistically significant differenee in Bcl-2 staining was found in relation to the histologic grade （P=0. 016）. Bcl-2 expression was irrelated to several clinicopathologic features, including age, gender and gross type （P〉0.05）. The positive rate of Bcl-2 immunostaining was 34.78% （8/23） in patients with lymphytic metastasis, compared with 63.27% （31/49） in pationts without lymphytic metastasis （P = 0. 024）. Staining frequency for Bcl-2 in Duke stage D was significantly lower than those of Duke stages A, B, C （P〈0.05）. CONCLUSIONS： Bcl-2 participates in the carcinogenesis of colorectal carcinoma and is probably associated with histologic grade, lymphytic metastasis and Duke stage.