摘要
目的 同时测定通淋颗粒中槲皮素和山奈素的含量.方法 采用8PLC法,样品经80%甲醇超声,酸水解后,采用色谱柱C18柱(4.6mm×250mm,5μm),甲醇-0.4%磷酸(50:50)为流动相,流速:1.0ml·min^-1,检测波长360nm,柱温:30℃.结果槲皮素在0.128~2.304ug(r^2=0.9996)和山奈素在0.1255~2.259ug(r^2=0.9999)范围内均呈良好的线性关系,槲皮素和山奈素的加权回收率分别为99.93%(RSD-1.06%)和99.23%(RSD-0.75%)(n=6).结论 本方法操作简便、重复性好、结果准确可靠,可满足通淋颗粒的质量控制要求。
Objective To establish a method for meletin and kaempferide in Tonglin Keli, Methods Sample were dissolved in 80% methanol with ultrasonic process and hydrolyse 1 houe. The HPLC system consisted of ODS-C18, methanol:0. 4%H3P04(50:50) as mobile phase, flow rate of 1. 0ml ·min^-1 and column tempreture of 30℃.The eluate was detected at 360 nm. Results The linerar ranges of meletin and kaempferide were 0.128-2.304 μg (r^2=0. 9996) and 0.1255-2.259μg (r^2=0. 9999),respectively, The mean recoveries of meletin and kaempferide were99.93% with RSD of 1.06% and 99.23% with RSD of 0.75% (n=6), respectively. Conclusion This method can be used for quality control of Tonglin Keli. It is simple rapid and accurate for simultaneous determination of two constituents in Tonglin Keli.
出处
《国际医药卫生导报》
2007年第4期86-88,共3页
International Medicine and Health Guidance News