大豆对SMVSC-13株系群的抗性遗传及基因定位的研究

INHERITANCE AND GENE MAPPING OF RESISTANCE TO SMV STRAIN GROUP SC-13 IN SOYBEAN

在接种SC-13株系群的情况下,鉴定了科丰1号×南农1138-2的P1、P2、F1、F2和184个重组自交家系(RIL)的抗性,结果显示,科丰1号(P1)与F1全部表现抗病,南农1138-2(P2)全部表现感病,表明抗性为显性;F2群体和184个重组自交家系出现抗感分离,卡方适合性检测表明F2群体抗感分离符合3∶1的比例,重组自交家系抗、感符合1∶1分离比率。表明对SC-13株系群的抗性由一对基因控制,以Rsc-13表示。利用已建立的遗传连锁图对Rsc-13进行了连锁分析,结果将抗病基因Rsc-13定位于N8-D1b+W连锁群上,并与抗性基因Rn1、Rn3、Rsc-7连锁。

The P,, P2, F,, F2 and 184 RILs of Kefeng No. 1 × Nannong1138-2 were inoculated with the SMV strain group SC-13 to evaluate their resistance. The results showed that Kefeng No. 1 and F1 were resistant to SC-13, Nannong 1138-2 was susceptible. F2 segregated in a 3 ： 1 ratio and the RILs in a 1 ： 1 ratio, which indicated a dominant gene controlled the resistance to SC-13. Through linkage analysis, the resistance gene Rsc-13 was mapped on the linkage group N8-D1b＋W and linked with Rnl .Rn3.Rsc-7.