影响大豆愈伤组织的诱导因素研究

RESEARCH ON AFFECTIVE FACTOR OF SOYBEAN CALLUS INDUCTION

比较MS、B5、N6三种基本培养基,发现MS适合作为诱导大豆愈伤组织的基本培养基。添加激素6-BA 0.5mg/L,2,4-D 2.0mg/L时愈伤组织诱导的效果最好,过高或过低的浓度激素均不利于愈伤组织的诱导和生长;不同外植体诱导愈伤组织的能力不一样,下胚轴诱导愈伤组织能力强于子叶;在所选择的六种大豆品种中,中黄13诱导愈伤组织的能力最强;光照条件为12h/d时有利于愈伤组织的诱导;培养基pH5.8-6.0时,愈伤组织的诱导率比较高。

Among the 3 media tested, MS was the most suitable for the callus induction of soybean. The medium added hormone with 0.5mg/L 6-BA and 2.0 mg/L 2,4-D was the best one for the induction of callus. Neither higher nor lower would suitable for the induction of callus. The effects of explants type on soybean callus induction were different, and the hypocotyls was more effecive than cotyledon on soybean callus induction. Among the 6 soybean types, zhonghuang13 was the most suitable for the callus induction of soybean. 12 hour light and 12 hour dark were benefit for the callus induction. The best pH for the callus induction and subculture was between 5.8 and 6.0.