大豆和玉米加工产品中外源转基因成分检测技术的建立

DETECTION OF GENETICALLY MODIFIED COMPONENTS IN PRODUCTS DERIVED FROM SOYBEAN AND MAIZE

采用PCR技术检测Ca MV35S启动子,并进一步通过PCR检测RoundUp Ready Soybean(RRS)和Bt176 Maxi maizer的特异性DNA片段,判断大豆和玉米加工产品中是否含相应转基因成分。在1份豆粕和豆腐样品中检测到了RoundUp Ready大豆特异性的498 bp片段,而在玉米粒样品中检测到了Bt176特异性转基因成分。PCR检测的灵敏度达到0.1%,稳定性良好。结果表明,PCR技术检测外源基因是灵敏和准确的,可以广泛地应用到转基因作物及其加工产品的转基因成分检测中。

The purpose of this study was to establish detection method on genetically modified components in products derived from soybean and maize using PCR technology. The presence of processed products were investigated by PCR detection of CaMV 35S promoter, and the presence of Round up Ready Soybean （RRS）, Bt176 Maximaizer in products derived from soybean and maize were further determined to detect their specific DNA fragments respectively by PCR. One bean cake sample and one bean curd sample were verified a 498bp RRS specific DNA fragment by PCR detection, Bt176 Maximaizer specific PCR products were obtained with the corn grain DNA samples used as PCR templates. The research also succeeded in settling 0. 1% precision degree of genetically modified organisms which ensured its stable property. The results showed that PCR analysis for the transgene was feasible and reliable. The PCR analysis of transgenic components could be widely used in various transgenic plants and their products.