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丹参对内毒素致急性肺损伤大鼠肺水通道蛋白-1的调节效应 被引量:8

Effect of Salvia Miltiorrhiza on Aquaporin-1 Expression in Rats Model of Lipopolysaccharideinduced Acute Lung Injury
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摘要 目的探讨丹参对内毒素(LPS)致急性肺损伤(ALI)大鼠肺水通道蛋白-1(AQP-1)的调节作用。方法SD大鼠48只,随机分为正常组、丹参注射液预保护组、丹参注射液7d治疗组、ALI7d模型组、丹参注射液8h治疗组、ALI8h模型组,每组各8只。分别于7d和8h后电镜及HE染色观察各组大鼠肺组织病理改变、肺湿/干重比(W/D)及肺脏水通透性的变化,免疫组化法及肺组织匀浆后Western blotting法检测AQP-1表达变化;测定血液氧分压PaO2、血液流变学、内皮素-1(ET-1)浓度。结果模型组均出现肺损伤症状。丹参注射液7d治疗组的肺水肿病理改变与ALI7d模型组比较明显减轻,血液流变学指标明显改善,但两组AQP-1表达均与正常组无明显差异(P<0.05)。丹参注射液8h治疗组和丹参注射液预保护组与ALI8h模型组比较,肺水肿病理改变明显减轻,血液流变学指标明显改善(P<0.05),其中丹参注射液预保护组AQP-1表达上调明显(P<0.01)。结论丹参可能通过调节AQP-1的表达改善水液代谢,改善ALI大鼠的血液流变学,从而减轻肺水肿状态,提示丹参新的药理效应依据。 Objective To investigate the effect of SM on aquaporin-1 (AQP-1) expression in rats with acute lung injury (ALl) induced by lipopolysaccharide (LPS). Method 48 SD rats were randomly divided into normal group, ALI 7-day group, ALI 8-hour group, SM prevention group, SM 7-day treatment group, and SM 8-hour treatment group, 8 rats per group. At 8 hours and 7 days after treatment, lung wet to dry weight ratio, lung pathology, electron microscope, AQP- 1 immunohistochemistry, and AQP- 1 Western blotting were performed. The situation of hemorheology and serum level of PaO2 and Endothelin- 1(ET- 1) were also detected. The model group rats an appeared ALI symptom. Compared with the 7-day ALI group, the degree of lung edema statement aUeviated in SM 7-day treatment group. But the expression difference of AQP-1 was not significant between the two groups (P〈0.05). Compared with the 8-hour .ALI group, the degree of lung edema statement aUeviated in SM 8-hour treatment group and SM prevention group .The expression of AQP- 1 was significantly increased in the group of SM prevention group (P〈0.01). Conclusion SM may improve the body fluid metabolism and the situation of hemorheology, and aUeviate the degree of the lung edema by regulating the expression of AQP- 1. It suggests a new pharmacological effect of SM.
出处 《中国中医药信息杂志》 CAS CSCD 2007年第3期30-32,共3页 Chinese Journal of Information on Traditional Chinese Medicine
基金 陕西省科学技术攻关项目(2005K10-G65) 西京医院创新基金(XJCX05M29)
关键词 丹参 急性肺损伤 脂多糖 水通道蛋白 血液流变学 Salvia miltiorrhiza acute lung injury lipopolysaccharide aquaporin-1 hemorheology
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  • 1Nuytinck HKS, Offermans XJMW,Kubat K, et al.Whole-body inflammation in trauma patients:an autopsy study.Arch Surg,1988;123:1519
  • 2Laurent T,Marker M,Flieder VV, et al.CD11b/CD18 expression adherence,and chemotaxis of granulocytes in adult respir- arory distress syndrome. Am j Respir Crit Care Med,1994;149:1534-1538
  • 3Wichterman KA,Baue AE, Chaudry IH. Sepsis and septic shock:a review of laboratory models and a proposal. J Surg Res,1980;29(2):189
  • 4Martin TR. Lung cytokines and ARDS. Chest,1999:116S2-S8
  • 5Chollet-Martinl S, Montravers P, Gibert C, et al. State of activation of polynuclear neutrophils and cytokines in acute respiratory distress syndrome in adults. Pathologic,1993;41 (8pt2):833-837
  • 6Ameison JC.Apoptosis and cell survival regulation: relevance to AIDS and Cancer.AIDS,1994;8:119-125
  • 7焦光宇,李尔然,于润江,刘冬娟.水通道蛋白1在肺组织中的表达[J].中华内科杂志,2001,40(1):45-46. 被引量:9

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