摘要
目的采用高效液相色谱法测定金茵颗粒剂中绿原酸的含量。方法Kroasil ODS柱(4.6mm×250mm);柱温:室温;流动相:0.3%乙酸-甲醇(74∶26);流速:0.9mL/min;检测波长:327nm。结果该方法的线性范围为65～325μg/mL,回归方程为:A=24239869C-31292,r=0.9993;平均回收率为100.10%,RSD=0.87%(n=6)。结论该方法可作为金茵颗粒剂的质量控制方法。
Objective To establish a HPLC method to determind the content of chlorogenic acid in Jinyin Granules. Method The mobile phase was 0.3%HAc-Methonal （74 ： 26）. The UV detection wavelength was 327 nm. The mobile speed was 0.9 mL/min. Separation column was Kroasil ODS （4.6 mm×250 mm）. A good linearity was obtained in the range of 65-325 μg, regression A=24239869C-31292, r=0.999 3. The average recovery was 100.10%, RSD was 0.87% （n =6）.Conclusion The method was good for determining chlorogenic acid in Jinyin Granules.
出处
《中国中医药信息杂志》
CAS
CSCD
2007年第3期43-44,共2页
Chinese Journal of Information on Traditional Chinese Medicine
基金
黑龙江省普通高校骨干教师创新能力资助计划项目(1053G004)
