淡竹叶中总黄酮提取工艺研究

Study on Extraction Process of Total Flavonoids in Lophatherum Gracile Brongn

目的用正交试验法优选淡竹叶中总黄酮的提取工艺。方法以20g原药材的总黄酮提取量为评价指标,选择乙醇用量(倍)、提取时间(h)、提取次数(次)为考察因素,采用正交试验法L9(34)确定醇提取淡竹叶中总黄酮的最佳工艺。结果最佳提取工艺条件为75%乙醇18倍量提取1.0h,共提取1次,乙醇用量为显著影响因素。结论首次进行了淡竹叶总黄酮正交提取工艺的研究,结果比较可靠。

Objective To study the optimum ethanol extraction process of total flavonoids in Lophatherun gracile Brongn. Methods The extraction of total flavonoids was choosen as the assessment index. Ethanol volumn extracting time and times were the studing factors. The optimum ethanol extraction process was selected with the orthogonal design. Results The optimum ethanol extracting process conditions were as follows： adding 18 fold of 75% ethanol, extracting for 1.0 h and one in all. Ethanol dosage was the predominant influencing factor. Conclusion This optimum extradion process of total flavonoids in Lophatherum grade Brongn is reliable.