包头地区汉族寻常型银屑病患者HLA-DQA1*0104等的检测

Detection of HLA-DQA1*0104 Allele of Psoriasis Vulgaris in the Han Nationality From Baotou Area

目的探讨包头市汉族寻常型银屑病患者与HLA-DQA1*0104等位基因的相关性。方法采用聚合酶链反应-序列特异引物(Polymerase chain reaction sequence specific primers,PCR-SSP)法检测75例寻常型银屑病患者及75例健康对照的等位基因频率,并相互比较。结果①HLA-DQA1*0104与包头市汉族寻常型银屑病患者具有明显的相关性(P<0.05,OR=3.45)。②HLA-DQA1*0104在Ⅰ型、Ⅱ型寻常型银屑病患者中分布无差异(χ2=0.076,P>0.05)。③HLA-DQA1*0104在有家族史和无家族史的患者分布有差别(P<0.05,OR=4.48)。结论①HLA-DQA1*0104可能是寻常型银屑病易感基因或与易感基因相连锁。②有家族史和无家族史寻常型银屑病患者在其遗传背景上存在有差异。

Objective To study the association of HLA-DQA1 ＊ 0104 allele with genetic susceptibility to psoriasis vulgaris （PV） in the Han nationality from Baotou. Methods Using high-resolution polymerase chain reaction amplification with sequence specifc primers（PCR-SSP） ,we detected the distribution frequencies of HLA-DQA1 ＊ 0104 allele among 75 patients with PV and 75 healthy controls, and compared with each other. Results ①HLA-DQA1 ＊ 0104 allele was markedly correlated psoriasis vulgaris from Baotou （ P 〈 0.05, OR = 3.45 ）. ②There was no difference of psoriasis vulgaris （ X^2 = 0.076, P 〉 0.05 ）. ③About HLA-DQA1 ＊ 0104 allele, there was markedly different distribution between psoriasis vulgaris patients with and without family history. Conclusion ①HLA-DQA1 ＊ 0104 may be susceptible gene. ②There may be a difference in genetic background between psoriasis patients with and without family history.