3种中药复方对脑缺血再灌注大鼠脑能量代谢相关酶影响的比较研究

Comparative Study on Effects of Three Traditional Chinese Medicinal Compounds on Energy Metabolism Related Enzymes in Cerebral Tissue of Rats after Focal Cerebral Ischemia and Reperfusion

目的比较3种中药复方对脑缺血再灌注大鼠脑能量代谢相关酶动态的影响。方法采用线栓法制作大鼠局灶性脑缺血再灌注模型,随机分为假手术组、模型组、益气活血方组、镇肝熄风汤组和星萎承气汤组,分别给予相应的药物灌胃。采用密度离心及差速离心法提取脑组织线粒体用于琥珀酸脱氢酶(SDH)活性测定,采用化学比色法测定脑组织匀浆Na^+ -K^+ -ATPase、肌酸激酶脑型同工酶(CK-BB)活性。结果模型组再灌注各时间点脑组织SDH、Na^+ -K^+ -ATPase活性显著降低,CK-BB活性显著升高(均P＜0．01); 48h和72h时各药物组SDH、Na^+ -K^+ -ATPase活性显著升高,24h时仅镇肝熄风汤组Na^+ -K^+ -ATPase活性显著升高;各药物组CK-BB活性均显著降低(P＜0．05);24h以镇肝熄风汤组为优,48h以星萎承气汤组为优,72h以益气活血方组为优。结论3种中药复方可以通过升高SDH、Na^+ -K^+ -ATPase活性,降低CK- BB的活性抗脑缺血再灌注损伤,24h镇肝熄风汤作用效果最佳,48h星蒌承气汤作用效果最佳,72h益气活血方作用效果最佳。

Objective To compare the effects of three traditional Chinese medicinal compounds on energy metabolism related enzymes in cerebral tissue of rats after focal cerebral ischemia and reperfusion （I/R）. Methods The local cerebral I/R model was established by ligation of the middle cerebral arteries （MCA）. The animals were divided into the sham-operative group, the model group, the Yiqi Huoxue Recipe （YHR） group, the Zhengan Xifeng Decoction （ZXD） group and the Xinglou Chengqi Decoction （XCD） group. The mitochondria in brain tissue was obtained by density-centrifugation and differential centrifugation, then the activities of succinate dehydrogenase （SDH）, Na^＋-K^＋-ATPase, creatine kinase-BB （CK-BB） in homogenate of brain tissue were measured by chemical chromometry. Results Activities of SDH and Na^＋-K^＋-ATPase were lower and that of CK-BB was higher in the model group than those in the sham -operative group at all time points after I/R （P〈 0.0l）. Compared with those in the model group, activity of Na^＋ -K^＋ -ATPase was higher only in the ZXD group at 24 h after I/R, while at 48 h and 72 h after I/R, activities of both SDH and Na^＋ -K^＋ -ATPase were higher in all the treatment groups. As for the activity of CK-BB, it was lower in all the treatment groups （P 〈 0.05）. The optimal effect was shown in the ZXD group at 24 h, in the XCD group at 48 h, and in the YHR group at 72 h after I/R. Conclusion The three traditional Chinese medicinal compounds could reduce pathologic injury after focal cerebral I/R in rats by promoting activity of SDH and Na^＋ -K^＋ -ATPase and inhibiting that of CK-BB, the optimal effect of ZXD was shown at 24 h after I/R, that of XCD at 48 h after I/R and of YHR at 72 h after I/R.