CO_2浓度倍增对AM真菌及其对绿豆接种效应的影响

Effects of Doubled CO_2 on AM Fungi and Inoculation Effects on Green Gram(Phaseolus radiatus L.)

在试验室CO2培养箱中模拟CO2浓度倍增环境,初步研究了CO2浓度倍增对AM真菌GlomuscaledoniumNicoison&Gerdemann及其对绿豆(PhaseolusradiatusL.)接种效应的影响。结果表明,绿豆生长14d时,CO2浓度倍增对AM真菌及绿豆生长没有显著影响。绿豆生长28d和42d后,CO2浓度倍增显著增加了AM真菌的侵染率和产菌丝量;接种AM真菌增加了地上部生物量,且在正常CO2浓度条件下达到显著水平;接种AM真菌增加了地下部生物量,且在CO2浓度倍增条件下作用显著。绿豆生长28d和42d后,CO2浓度倍增条件下,接种AM真菌增加了根冠比,且在28d时达到显著水平。而在正常CO2浓度条件下接种AM真菌对根冠比的作用不明显。绿豆生长42d后,不论是在CO2浓度倍增条件下,还是在正常CO2浓度条件下,接种AM真菌均增加了地上部分的总碳、氮含量。AM真菌和CO2浓度倍增的交互作用仅在28d时对地上部分总碳、氮含量、地下部分生物量和根冠比的作用达到显著水平。

Effects of doubled CO2 on AM fungi（Glomus caledonium Nicoison＆ Gerdemann）and its inoculation effects on green gram （Phaseolus radiatus L.）were investigated in CO2 growth chamber. Doubled CO2 had no significant effect on AM fungi and its effectiveness at 14 day after sprouting of green gram. At 28 and 42 day after sprouting, doubled CO2 increased AM colonization and hyphal length significantly; AM fungi increased shoots biomass （significant under ambient） and roots biomass （significant under doubled CO2） of green gram. The ratio of root/ shoot increased significantly with AM fungi under doubled CO2 at 28 day after sprouting, but the effect of AM fungi on ratio of root/shoot under ambient was little. At 42 day after sprouting, AM fungi increased the total content of carbon and total nitrogen in shoots under both ambient and doubled CO2. The interaction between AM fungi and doubled CO2 was not significant except for total carbon in shoots, total nitrogen in shoots, roots biomass and root/shoot at 28 day after sprouting.