RTN4-C相互作用蛋白的筛选与鉴定

Screening and Identification of the RTN4-C Interactive Proteins

我们的前期工作发现过表达RTN4-C基因能够抑制肝癌细胞株SMMC7721的生长并且促进其发生凋亡.为了进一步了解RTN4-C促进凋亡的机制,本文采用酵母双杂交的方法从小鼠7 d胚胎文库中筛选其相互作用的蛋白.最终筛选到其同源家族成员RTN3.进一步采用间接免疫荧光和及构建的荧光载体表明,RTN4-C和RTN3蛋白在HEK293和Hela细胞中发生共分布.本研究为进一步阐明RTN4-C抑制细胞生长和促进细胞凋亡的机制提供了新的依据.

Our previous study showed that overexpression of the RTN4 - C might inhibit SMMC7721 cell growth and promote cell apoptosis. To investigate the mechanism of RTN4 - C inhibition of cell growth and pro-apoptosis, the yeast two-hybrid method was adopted. By screening the mouse 7 - day Embryo MATCHMAKER cDNA Library, we obtained the interacted protein RTN3, which has been reported to pro-apoptosis. Our further study indicated that RTN4 - C and RTN3 colocalized in HEK293 and Hela cells using immunofluorescence and fluorescence vectors. The present study could be useful to clarify the mechanism of RTN4 - C inhibition of cell growth and pro-apoptosis.