中药白头翁中23-羟基白桦酸对照品的制备及鉴定

Preparation of 23-hydroxybetulinic Acid from the Roots of Pulsatilla chinensis

目的:研究中药白头翁中对照品23-羟基白桦酸的制备方法。方法:以酸水解的方法从白头翁药材中提取总三萜,以硅胶柱层析和重结晶的方法从总三萜中分离、制备对照品,采用波谱方法对其进行结构鉴定。结果:从白头翁总三萜中分离出23-羟基白桦酸对照品。结论:该对照品可作为控制白头翁总三萜制剂质量的指标成分。

Objective： To isolate and purify high purity 23-hydroxybetulinic acid from the roots of PulsatiUa chinensis. Methods： By acid hydrolysis, silica gel column chromatographies and recrystalization methods, 23-hydroxybetulinic acid was obtained from the roots of P. chinensis. Results： High purity 23-hydroxylbetulinc acid was isolated from the roots of P. chinensis, the contents is over 98.0%. Conclusion： The preparation process is feasible and convenient. High purity 23-hyroxybetulinic acid could be used in the quality control of the materials and medicament of 23-hydroxybetulinic acid.