基质金属蛋白酶3在创伤愈合中作用的实验研究

Experimental study on effects of matrix metalloproteinase 3 involving in wound healing

目的探讨基质金属蛋白酶3在创伤愈合过程中的变化及其意义。方法取伤后1d伤口液作为创伤修复启动微环境，刺激真皮多能干细胞，24h后检测细胞表达MMP3含量的变化。同时制作单纯创伤和难愈创伤动物模型．通过免疫组化和图像分析方法观察伤后不同时相伤口组织MMP3含量变化。结果创伤早期伤口液作用真皮多能干细胞后表达MMP3显著增加。正常大鼠皮肤组织成分可见一定量的MMP3表达，阳性细胞主要为表皮细胞、毛囊外根鞘细胞、真皮成纤维细胞、血管内皮细胞等。单纯创伤大鼠伤后各时相点MMP3表达均显著增加，特别是以真皮组织增加更为明显．其峰值在伤后5，7d。难愈创伤组大鼠伤I：I局部MMP3含量在伤后各时相点亦显著增加，但与单纯创伤组相比，其含量在伤后3、5、7d均显著减少，尤以真皮组织表达减少更为明显，并且MMP3表达峰值延迟，为伤后10d。结论MMP3是一种重要的创伤愈合调节分子；通过高表达MMP3可能是真皮多能干细胞参与创伤修复的途径之一。

Objective To explore the changes and significances of matrix metalloproteinase 3 （MMP3） during wound healing. Methods Dermal pluripotent stem cells （DPSCs） were routinely isolated, purified and expanded. Wound fluids of 1st day were collected as wound microenvironment,and the responses of DPSCs to wound fluids were investigated and the expression of MMP3 protein in DPSCs were determined by immunohistochemistry staining. Meanwhile, animal models were conducted by cutaneous cutting and suture, which were respectively assigned to impaired wound group or simple wound group according to the combination with or without whole-body irradiation. Then MMP3 contents in wound sites were also determined by immunohistochemistry assay and image analysis. Results The expression of MMP3 in DPSCs increased significantly after stimulation by wound fluids. MMP3 contents in rats of simple wound group increased significantly, especially in the dermal tissues,and the peak time was the 5th - 7th day after wounded. MMP3 contents in rats of impaired wound group were significantly less than those of simple wound group, and the peak time was also delayed, which was the 10th day. Conclusions MMP3 may be an important molecule involved in wound healing. DPSCs may also participate in the processes of wound repairing via highly expressed MMP3.