狼毒抑制降低癌细胞恶性表型小鼠体内转移及彗星电泳实验研究

Experimental Study on Radix Euphorbiae Fischianae Extract Inhibiting or Decreasing Maliganant Cell Phenotype to Transfer in Rat and Comet Electrophoresis.

目的分析狼毒水提液抑制癌细胞增殖凋亡的作用机理。方法观察狼毒抑制肿瘤细胞在小鼠体内的转移能力，放射线照射引起的正常肝细胞彗星电泳尾长度，肝肿瘤细胞表面绒毛状突起消失及由环磷酰胺引起的微核率增高降低情况。结果能显著的抑制B16-F10肿瘤细胞在小鼠体内的转移，彗星电泳尾缩短改变，肿瘤细胞恶性表型降低，能够降低由环磷酰胺引起的微核形成率。结论狼毒可通过多种机制抑制肿瘤的生长或转移，不仅是一种抗肿瘤药物，而且还具有良好的放射防护和免疫调节功能。

Objective To analysis the inhibitory, effect of roliferation and apoptosis of neoplasm and its mechanisms. Methods It was investigated that Radix Euphorbiae Fischianae extract inhibit neoplasm to transfer in rat,that the langth of radiated normal heptocyte after comet electrophorsis was measured, that thequantity of the surface villous towers of heotocyte increased or decreased, and that the mironucleus rat led by cyclophosphamide changed.Results Radix Euphorbiae Fischianae extract can greatly inhibit the B 16-F10type tumur cel to transfer.The langth of comet electrophorsis was shortened.The malignant phenotype and the micronucleus rat were decreased.Conclusion Radix Euphorbiae Fischianae extract can inhibit the cancer cell to transfer by the means of manymechanisms. It not only is antitumorigensis medicine,but also can improve the immunization.