山西不同品种大豆贮藏蛋白提取及亚基分析

The Extraction and Subunit Analysis of Storage Protein of Different Soybean Cultivars in Shanxi Province

用不同盐缓冲溶液分别提取大豆贮藏蛋白,对提取效果分析比较,结果表明,Tris-HCl(pH 7.8)缓冲溶液的提取方法最为合适;在确定提取方法的基础上,对山西不同品种大豆的贮藏蛋白进行了SDS-PAGE分析,电泳图谱显示,不同品种间贮藏蛋白亚基在数量和含量上有较大差别。

To study the subunit of soybean storage protein, storage protein extracted by different buffer from defatted soybean were analyzed by SDS-PAGE electrophoresis. The results showed that duilt Tris-HCl （pH7.8） buffer was the best choice to extract storage protein. Storage protein of different soybean cultivars in Shanxi province were analyzed by SDS-PAGE electrophoresis, SDS-PAGE Patten show that there have differences in the number and content of subunit.