摘要
目的:观察结缔组织生长因子(connective tissue growth factor,CTGF)在人及大鼠纤维化肝组织中的表达,探讨CTGF及其介导的生物学信号在肝纤维化发生中的作用机制。方法:雄性SD大鼠32只,皮下注射CCl_4制备大鼠肝纤维化模型,分别于注射后1、4、8用处理动物并收集肝组织标本;44例人肝组织(12例正常肝组织、32例慢性病毒性肝炎和肝硬化组织)来自外科手术切除的诊断明确的病理存档标本。采用免疫组化方法对检测肝组织中CTGF的表达及分布。结果:免疫组化显示．CTGF主要表达于大鼠肝星状细胞(HSC)及肝细胞胞质中。CCl_4注射诱导后,大鼠肝组织中CTGF的表达较正常对照明显增强(P＜0．01或P＜0．05),且CCl_4注射1、4、8周组肝组织中CTGF的表达强度呈明显递增的趋势(P＜0．01或P＜0．05)。在人肝纤维化组织中,CTGF亦主要表达于HSC胞质及部分肝细胞胞质中,CTGF表达水平显著高于人正常肝组织(P＜0．01)。结论:CTGF作为一种促纤维化因子,其过表达主要促进HSC的增殖活化,促进细胞外基质的形成,从而参与肝纤维化的发生、发展。
Objective..To explore the role of Connective tissue growth factor（CTGF）in the mechanism of liver fibrosis via investigating the expression of CTGF in human and rats＂ fibrotic liver tissue. Methods ： Liver fibrosis models of rats were made by subcutaneously injecting with CCl4. Thirty two male SD rats （weight 250-300g） were randomly scarified at 1st ,4th and 8th after injection of CCl4, respectively, and their liver tissues were used for examining CTGF by immunohistochemistry stain. On the other hand, immunohistochemistry stain was used to detect the expression of CTGF in 32 human liver cirrhosis and 12 normal liver tissues too. Results： The expression of CTGF in rats after injection with CCl4 were found chiefly in hepatic stellate cells（HSC） and hepatocytes and all significantly higher than those in normal rats（P〈0.01 ,〈0.05）. Moreover, it presented with progressive tendency for the expression of CTGF in rats respectively at 1^st ,4^th and 8^th week after injection with CCl4 （P〈 0.01 ,〈0.05）. It presentsed with significantly progressive tendency for the expression of CTGF in human normal tissues and liver cirrhosis（P〈0.01） too. Conclusion：The overexpression of CTGF maybe accelerate the procedure of liver fibrosis via enhancing HSC proliferation and the ECM increase, and play an important role in liver fibrogenesis.
出处
《中国临床医学》
北大核心
2007年第1期10-12,共3页
Chinese Journal of Clinical Medicine
基金
广西壮族自治区科学研究与技术开发项目(0448030)
关键词
结缔组织生长因子
肝纤维化
肝星状细胞
Connective tissue growth factor
Liver fibrosis
Hepatic stellate cell
