大肠癌中Survivin、PTEN、p16的表达及其临床意义

The Expression and Clinical Significance of Survivin,PTEN and p16 in Colorectai Cancer

目的:探讨Survivin、PTEN、p16基因在大肠癌中的表达及其临床意义。方法:利用免疫组织化学SP法检测43例结直肠癌和12例正常结直肠粘膜组织中以上3种基因的表达产物。结果:43例结直肠癌组织中Survivin基因表达23例,癌旁正常直结肠组织中无Survivin基因的表达,Survivin的表达与结直肠癌的组织分型、浸润深度、Dukes'分期无明显相关性(P＞0．05),与结肠癌的转移有相关性(P＜0．05)。而PTEN,p16与大肠癌的分化程度、浸润深度、临床分期、淋巴结转移有关(P＜0．05)。结论:Survivin、PTEN、p16的异常表达在大肠癌发生、发展过程中起重要作用,联合检测Survivin、PTEN、p16表达水平有可能作为反映大肠癌进展和预后的生物学指标。

Objective： To investigate the expression of survivin, PTEN and p16 in their coloncarcinoma and the clinical significance. Methods：Immunohistochemical SP method was used to detect the expression of 3 proteins in 12 cases of normal colon tissue and 43 cases of coloncarcinoma. Results： We found that survivin in tissues of 23 cases of coloncarcinoma had positive expression, survivin was not expressed in normal tissue, over expression of survivin had no relation with histological type and Dukes classification,but it had obviously association with lymph node metastasis （P〈0. 05） ,the expression of PTEN and p16 were correlated with histological type,Dukes classification and lymph node metastasis. Conclusion： Abnormal expression of survivin, PTEN and p16 play an important role in occurrence and progression of large intestin carcinoma, associating survivin with PTEN and p16 may be amolecular-biological indicator in forecasting the progress and prognosis of colorectal carcinoma.