单纯空腹血糖受损伴正常白蛋白尿患者尿液蛋白质谱分析

Urinary Protein Profile of Impaired Fasting Glucose Subjects with Normal AIbuminuria

目的:研究单纯空腹血糖受损(IFG)伴正常白蛋白尿患者尿液蛋白质谱。方法:应用表面加强激光解吸电离-飞行时间-质谱(SELDI—TOF—MS)技术,选用IMAC—Cu^(2+)蛋白质芯片,优化技术参数,对尿白蛋白肌酐比值正常的20例正常人,22例单纯空腹血糖受损患者的尿液标本进行检测,应用Biomarker Wizard分析IFG患者尿液蛋白质谱。结果:IMAC—Cu^(2+)芯片在激光强度230,灵敏度为9的条件下可获得较理想的图谱。平均M/Z2200、2353、2443、2579、2755、2797、2910、3003、3097、3606、3645、4029、5083和5511处质峰强度差异在2倍以上(P＜0．01)。IFG组在M/Z 5083处下调,其余则上调。结论:SELDI—TOF—MS技术是一种快速、简便和高通量比较蛋白质组学分析方法。尿白蛋白排量正常的单纯空腹血糖受损患者存在多种小分子蛋白质/多肽的排泄异常。

Objective：Surface enhanced laser desorption/ionization time-of-flight mass spectrometry（SELDI-TOF-MS） is a novel method for biomarker discovery that can provide a rapid protein expression profile. The current study was undertaken to investigate urinary protein profile of isolated impaired fasting glucose patients with normal albuminuria. Methods： Urine of 20 NGT and 22 IFG without elevated ACR were analyzed using surface enhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS） and potential differences in protein or polypeptide profile were identified by Biomarker Wizard Software. Results： Protein profile of urine showed the best peak spectrum on IMAC-Cu^2＋ arrays by sensitivity of 9 and laser intensity of 230. The intensities of protein/polypeptide peaks appeared upregulated in IFG group at M/Z2200,2353,2443, 2579,2755,2797,2910,3003,3097,3606. 3645,4029 and 5511. but downregulated at M/ZS083. Conclusion： SELDI is powerful and high throughput in comparative proteomic analysis. Small molecular proteins and/or polypeptides excretion may abnormal in IFG with normal urinary albumin.