MMP-1/TIMP-1在膝关节滑膜皱襞中的表达及其意义

The Expression of MMP-1/TIMP-1 in Synoviai Plica of Knee Joint and Implications

目的:探讨病理性滑膜皱襞发病机制中有基质金属蛋白酶的参与对软骨破坏的影响。方法:关节镜检查确诊为病理性滑膜皱襞和正常滑膜皱襞,分别进行免疫组化染色,观察基质金属蛋白酶-1(MMP-1)和组织金属蛋白酶抑制因子-1(TIMP-1)的表达及分布。结果:MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达具有显著差异(P＜0．01．P＜0．05)。MMP-1在滑膜衬里层细胞、单核和淋巴细胞、血管内皮细胞和化生的软骨细胞呈阳性表达,而在正常滑膜皱襞组织中不表达。TIMP-1只在滑膜衬里层细胞和少量的成纤维细胞有表达,而免疫组化显示MMP-1阳性细胞数多于TIMP-1和着色强度强于TIMP-1。结论:病理性滑膜皱襞可产生MMP-1、TIMP-1,而且两者分布不平衡,可能是导致软骨破坏的生物学因素之一。

Objective： To explore on pathogenesis of pathologic synovial plicae damage to cartilage due to matrix metalloproteinase. Methods： The immunohistochemical method were used to observe expressions and distributions of matrix metalloproteinase-1 （MMP-1） and tissue inhibitor of metalloproteinase-1（TIMP-1 ）in the pathologic synovial plicae and normal synovial plicae of knee under arthroscopy. Results：The positive expressions of MMP-1 and TIMP-1 had significant difference between the experiment and control group（P〈0.01, P〈0.05）. The expression of MMP-1 was positive in synovial lining cell, monocyte, fibroblast cell,endothelial cell in small vessel and chondrocyte. The TIMP-1 expression was detected in the synovial lining cells and a small quantity fibroblast cells. Conclusion：The pathologic synovial plicae can produce MMP-1 and TIMP-1. Both of unbalance distributions perhaps are the biological pathogenesis of cartilage destruction.