广东国际旅行人员HIV-1基因亚型分析

Analysis of Subtype of HIV-1 Strains among Internotional Travelers at Guangdong Port

〔目的〕通过对广东口岸国际旅行人员中HIV感染者的亚型分析,监测HIV毒株的流行状况及其变化。〔方法〕从22份HIV-1抗体阳性样品中提取RNA或DNA,逆转录后或直接巢式PCR扩增HIV env及gag基因,对PCR产物进行核苷酸序列测定和分析,确定基因亚型。〔结果〕22份样品中共发现8种HIV-1亚型和重组型,其中CRF-BC重组型8份,CRF01-AE重组型4份,C亚型2份,B亚型2份,A1、G、J亚型及CRF02-AG重组型各1份,2份样品分型不明确。这些不同亚型的感染者来自包括中国在内的8个不同国家。〔结论〕国际旅行人员中HIV-1亚型和重组型种类复杂多样,是我国HIV毒株输入的主要来源。监测国际旅行人员中HIV感染者基因型分布及其变异,及时发现新型毒株对于艾滋病的预防和控制十分重要。

Objective To monitor the epidemic status of HIV strains through analyzing HIV subtype among HIV-infected international travelers at Guangdong frontier port. Methods RNA or DNA was extracted from 22 HIV-1 positive samples, HIV env and gag genes were amplified by RT and nested-PCR using specific primer pairs and sequenced directly. The acquired sequences were analyzed to determine the HIV subtype. Results There were 8 kinds of HIV-1 subtypes and recombinants in 22 samples,of which, 8 cases of CRF- BC, 4 cases of CRF-01AE, 2 cases of C, 2 cases of B and 1 cases of A1, G, J and CRF02-AG, 2 cases could not be determined. These HIV infectors came from 8 different countries including China. Conclusion There are kinds of HIV-1 subtypes and recombinants among international travelers,which is the main source of imported HIV strain. It isvery important for HIV/AIDS prevention and control to surveil of the genotypes and the variation of HIV strains among the international travelers and find new subtypes.