健脾清化法对沙土鼠HP感染的根除及胃黏膜NO含量的影响

The Influence of Invigorating Spleen and Eliminating Damp-Heat Therapy on HP Eradication on Mongolia Gerbils,and on NO Content in the Stomach Mucosa

目的探讨健脾清化法对蒙古沙土鼠HP感染的根除作用及对胃黏膜NO含量的影响。方法蒙古沙土鼠40只,随机分为正常对照组、病理模型组、阳性药物对照组、健脾清化汤组各10只。实验前进行13C-UBT。除正常对照组外,其余三组采用灌胃法复制HP感染模型,并分别予胃三联及健脾清化汤治疗。停药后4周进行13C-UBT并提取胃黏膜,供脲酶试验、细菌学检查及黏膜中NO的测定。结果经过治疗后HP感染沙土鼠13C-UBT测值除病理模型组外均有不同程度的下降,与治疗前比较差异显著;同时脲酶试验及细菌学检查为阴性,胃黏膜NO含量均有相应程度的下降,与病理模型组比较差异显著。结论健脾清化汤和胃三联治疗对HP感染沙土鼠有明显的根除作用。健脾清化汤对沙土鼠HP感染后NO含量的增加有一定的抑制作用。

Objective： To discuss the influences of Invigorating Spleen and Eliminating Damp - Heat （ISED） Therapy on HP eradication on Mongolia gerbils, and on NO content in the stomach mucosa. Methods： Randomly divided 40 Mongolia gerbils into normal control group, pathologic model group, drug positive group, and ISED group, 10 gerbils each. Performed ^13 C - UBT before experiments, intragastrically fed them, except of the normal control group, toduplicate the HP infectious models, and then treated them by gastral triple therapy and ISED soup. After 4 -week drug withdrawal, we tested ^13 C -UBT again and extracted stomach mucosa for urea enzyme test, bacteriology, NO content tests in mucosa. Results： After treatment, ^13 C - UBT of the HP infectious Mongolia gerbils, except of the pathologic model group, decreased on different levels, with significant deviation compared to that before treatment. Meanwhile, urea enzyme and bacteriologic test negative, NO contents in stomach mucosa dropped correspondingly, significantly deviated to the pathologic model group. Conclusion： ISED Decoction and gastral triple therapy displayed distinct HP eradication effect on HP infectious Mongolia gerbils. And ISED Decoction acted some inhibitory effect on NO contents raise after gerbils infected by HP.