含益生菌的肠内营养对重型颅脑损伤大鼠超氧化物歧化酶及丙二醛的影响

Influence of enteral nutrition supplemented with probiotics on SOD and MDA of rats with severe craniocerebral injury

[目的]探讨含益生菌的肠内营养对重型颅脑损伤大鼠血清和胃黏膜超氧化物歧化酶(SOD)和丙二醛(MDA)的影响。[方法]建立重型颅脑损伤大鼠模型,将57只SD大鼠随机分为益生菌+肠内营养组(EC组)、肠内营养组(EN组)、创伤对照组(I组)和正常对照组(C组),于喂养后第1天、第3天、第5天取标本,采用黄嘌呤氧化酶法和比色法分别检测血清和黏膜中的SOD和MDA含量。[结果]EC组、EN组、I组与C组相比,血清和胃黏膜SOD显著下降(P<0.05或P<0.01),MDA升高明显(P<0.01);EC组、EN组与I组比较,血清与胃黏膜SOD活性明显增强而MDA含量显著下降(P<0.01或P<0.05);EC组在喂养第3天、第5天与EN组相比,血清和胃黏膜中SOD活性明显增强而MDA显著下降(P<0.05或P<0.01)。[结论]重型颅脑损伤大鼠补充含益生菌的肠内营养,可显著增强血清和胃黏膜中SOD活性,从而促进清除血清和胃黏膜中MDA的能力。

Objective： To probe into the influence of enteral nutrition supplemented with probiotics on serum and stomach mucosa superoxide dismutase （SOD） and malonaldehyde （MDA） levels of rat with severe craniocerebral injury. Methods： To prepare the rat model of severe craniocerebral injury, a total of 57 SD rats were randomly divided into EC group （enteral nutrition plus probiotics）, EN group （enteral nutrition）, injury group （groupⅠ） and normal control group （group C）. Specimens were collected on the first day, the third day and the fifth day after feeding respectively. Then contents of SOD and MDA in both serum and gastric mucosa of all rats were detected by xanthine oxidase method and colorimetric method. Results： Compared with group C, serum and gastric mucosa SOD levels of rats in EC group, EN group and group Ⅰ were significantly decreased （ P 〈 0.05 or P 〈 0.01 ）. However, MDA levels of rats in EC group, EN group and group Ⅰ were significantly elevated than that of group C rats（P〈0.05 or P〈0.01）. Compared with rats in group Ⅰ, SOD activities in serum and gastric mucosa were apparently higher and MDA contents were significantly lower than that of rats in EC group and EA group （P 〈 0.05）. Compared with rats of EN group on the third day and fifth day＇s feeding after injury, SOD activities in serum and gastric mucosa of rats in EC group were apparently higher and MDA were significantly lower （P〈0.05 or P〈0.01 ）. Conclusion： Enteral nutrition supplemented with probiotics could significantly strengthen the SOD activities in serum and gastric mucosa of rats with severe craniocerebral injury and promote their ability of clearing MDA in serum and gastric mucosa.