番红花球茎的快速高频诱导

Rapid and High Frequent Induction of Saffron Corms

以体外培养的脱毒番红花芽为材料,考察了外源激素及活性炭对球茎诱导的作用.外源激素水平对球茎诱导有重要影响,较低的激素浓度下球茎诱导时间较短,较高的激素浓度下诱导出的球茎较大,但诱导时间延长.培养基中添加活性炭后,由于活性炭的吸附作用降低了游离激素水平,显著缩短了球茎诱导时间,后期激素的缓慢释放提高了球茎质量.在优化的1/2MS+5mg/LNAA+5mg/L6-BA+0.5g/LAC培养基中,5周球茎诱导率高达85.7%,球茎均重0.53g,满足移栽要求.

The effects of exogenous plant hormones and activated carbon （AC） on the development of saffron corms in vitro were studied. Both hormone concentration and AC level influenced corm induction. When 1/2 Murashige and Skoog medium was supplemented with low concentration of hormones, corms were induced quickly while big corms formed slowly at high concentration of hormones. The addition of AC decreased the concentration of free hormones and the time of corm development significantly, while the slow release of hormones raised corm size. In the medium with optimal 0.5 g/L AC, high induction rate （85.7%） and big corms （0.53 g average weight） were obtained in 5 weeks when 5 mg/L naphthalene acetic acid and 5 mg/L 6-benzylaminopuine were added to the medium.