生脉注射液对家兔心肌缺血再灌注损伤的保护作用

Protective Role of Shengmai Injection on Reperfusion Injury of Myocardial Ischemia in Rabbits

目的:探讨生脉注射液对家兔心肌缺血再灌注损伤的保护作用。方法:将30只家兔随机分为非缺血对照组(Ⅰ组)、缺血再灌注组(Ⅱ组)及缺血再灌注生脉注射液保护组(Ⅲ组),观察缺血30min和再灌注60min肌酸磷酸激酶(CPK)、肌酸磷酸激酶同工酶(CK-MB)、丙二醛(MDA)及超氧化物歧化酶(SOD)的影响,以及电子显微镜下家兔心肌超微结构的变化。结果:Ⅲ组家兔血清CPK、CK-MB含量、MDA值显著低于Ⅱ组(P<0.01),心肌SOD活性显著高于Ⅱ组(P<0.01),Ⅲ组家兔心肌超微结构较Ⅱ组明显改善。结论:SMI具有清除氧自由基,减少心肌酶的释放,明显改善缺血再灌注损伤心肌的超微结构。

Objective： To discuss the protective effect of Shengmai Injection on myocardium of rabbits with ischemia reperfusion injury. Methods： Thirty healthy rabbits were divided into 3 groups, including non- ischemia group （Ⅰ group）, ischemia reperfusion group （Ⅱ group）, and ischemia reperfusion with SMI treatment group （Ⅲ group）〈 n = 10）. The content of blood serum myocardial enzyme（including Creatinine phosphokinase CPK, CK-MB, Assay the activity of superoxide dismutase （SOD） and the contentration of malondialdehyde （MDA）. Observe the rabbit＇s ultrastructure of myocardium was detectded at the same time. Compare the different between the three groups. Results： As compared with Ⅱ group, the concent of blood serum myocardial enzyme and the contentration of MDA were decreased and the activity SOD were increased in m soup The ultrastructure of myocardium of ischemia reperfusion injury in Ⅲ soup was improved. Conclusion： SMI can enhance the activity of SOD and get rid of OFR. SMI can decrease the release of myocardial enzyme and improve the ultrastructure of the myocardium of ischemia reperfusion injury.