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诺卡氏菌属菌株04-5195发酵产物Amicoumacin B对BMP-2基因作用的研究 被引量：3

Study of the Effect of a Production of Nocardia 04-5195 Amicoumacin B on BMP-2 Gene
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摘要 目的研究Amicoumacin B对骨形态形成蛋白因子Ⅱ(BMP-2)基因的作用。方法构建以bmp-2启动子为作用靶点、荧光素酶为报告基因、通过测定模型荧光强度而间接反映药物上调活性的检测模型,研究诺卡菌属菌株04-5195发酵产物Amicoumacin B对BMP-2基因的作用。结果Amicoumacin B可上调BMP-2基因的表达。结论Amicoumacin B可由诺卡菌属菌株产生,并具有促进成骨细胞活性的潜能。 OBJECTIVE To investigate the effect of Amicoumacin B on bone morphogenetic protein-2 （BMP-2） gene. METHODS Establish detecting model targeting the BMP-2 promoter, in which the firefly luciferase reporter gene driven by the BMP-2 promoter of the mouse is used, to assess the effects on the promoter are by the luciferase activity. Using the model, we study the effect of a product of Nocardia 04-5195 Amicoumacin B on BMP-2 gene. RESULTS Amicoumacin B can enhance the expression of the BMP-2. CONCLUSION Amicoumacin B can be producted by Nocardia and has the potential of increasing osteoblast activity.
作者 杨兆勇 孙薇 杨隽 丁艳 司书毅 张月琴
机构地区 中国医学科学院医药生物技术研究所国家新药筛选中心
出处 《中国药学杂志》 CAS CSCD 北大核心 2007年第4期268-271,共4页 Chinese Pharmaceutical Journal
基金 国家自然科学基金资助项目(30100225)
关键词 诺卡氏菌属 Amicoumacin B 骨形态形成蛋白因子Ⅱ Nocardia Amicoumacin B bone morphogenetic protein-2
分类号 R966 [医药卫生—药理学]
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参考文献6

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同被引文献16

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中国药学杂志
2007年 第4期

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