应用荧光定量聚合酶链反应(FQ-PCR)检测孕妇巨细胞病毒感染

FQ-PCR and its application in detection of pregnant women CMV infection

目的探讨荧光定量聚合酶链反应对孕妇巨细胞病毒（CMV）感染的临床诊断价值。方法用FQ—PCR检测1016例孕妇尿的CMV—DNA，同时用ELISA检测血中CMV IgG和CMV IgM。对20例感染孕妇的胎儿进行了产前诊断。结果FQ—PCR检测出36例CMV—DNA阳性，阳性率为3．5％（36／1016）；阳性样本CMV—DNA在3．9×10^5～6．8×10^8拷贝／ml之间，平均为4．23×10^5拷贝／ml；ELISA检测CMV—IgG阳性率72．83％（740／1016）；CMV—IgM阳性率3．15％（32／1016），CMV—IgG阴性率24．02％（244／1016）。20例产前诊断者有4例发生了宫内传播。结论FQ—PCR对CMV感染的诊断有重要的作用。

Objective： To evuluate FQ - PCR on detection CMV - DNA infection in pregnant women. Methods： Urine CMV - DNA was detected by FQ - PCR and ELISA was used to screen for serum CMV - IgG and CMV - IgM in 1016 pregnant women. The 20 fetuses of infected of pregnant women were prenatal diagnosed by FQ - PCR. Results： The positive rate of CMV - DNA was 3.5% （36/1016）. The copies of CMV - DNA were 3.9×10^5 - 6. 8×10^8 copies/ml, average is 4. 23 ×10^5 copies/ml. The positive rate of serum CMV - IgG was 72. 83% （740/1016）. The positive rate of serum CMV -IgM was 3. 15% （32/1016）. The negative rate of serum CMV -IgG was 24. 02% （244/1016）. CMV intrauterine infection were eonsiderod in 4 eases. Conclusion： FQ -PCR is absolutely necessary methods for diagnosis CMV infection in pregnant women.