毁灭泰泽球虫Tyzzeria Perniciosa(Sporozoa:Eimeriidae)生活史各阶段的细胞化学研究:Ⅰ.多糖

CYTOCHEMISTRY STUDIES OF TYZZERIA PERMOIOSA IN THE BEIJING DUCK: Ⅰ. POLYSACCHARIDE

本实验分别用过碘酸——雪夫氏剂染色方法(PAS)和乌洛托品——硝酸银染色方法在光镜和电镜下检验毁灭泰泽球虫生活史各时期体内的多糖及其分布。实验结果表明,子孢子内、各代裂殖体和裂殖子内都含有多糖。大配子和合子内除含有多糖外还含有成囊颗粒。成囊颗粒的成分是糖蛋白。无性世代的滋养体和多核体内未检出多糖。早期配子细胞,小配子体和小配子内也未检出多糖。本实验证明,毁灭泰泽球虫体内的多糖系由其自身合成,并在其发育过程中消耗。

Periodic acid-Schiff's stain (PAS) and methenamine-silver method were used for detecting the presence and distribution of polysaocharide in different stages of the life cycle of Tyzzeria ferniciosa (Sporozoa: Eimeriidae).In the exgenous stage, the zygotes contained polysaccharide and the sporozoites also contained polysacoharide granules in their early stage of development, but these materials were depleted at the end of the development.All generations of schizonts and merozoites contained polysaccharide. The macrogametes were rich in polysaccharide. The experiment evidenced that these polysaccharide were elaborated by means of synthesis by the protozoa themselves and consumed as energy source during their movement and development. The macrogametes and zygotes possessed wall forming bodies of polysaccharide-protein ingredients.