溃疡性结肠炎患者基质金属蛋白酶-1表达测定的意义

Significances of matrix metalloproteinase - 1 expressions in patients with ulcerative colitis

目的检测基质金属蛋白酶-1（MMP-1）在溃疡性结肠炎（UC）患者结肠组织中的表达，探讨MMP-1在溃疡形成过程中的作用以及与病情严重程度之间的关系。方法采用逆转录-聚合酶链反应（RT—PCR）和免疫组织化学染色法在40例UC患者的结肠黏膜溃疡区、炎症区以及相对正常区取活组织在mRNA水平及蛋白水平上测定MMP-1表达，以10例正常人的结肠组织（正常对照组）作为对照，分析MMP-1的表达与病情严重程度之间的关系。结果在mRNA水平上，MMP-1在溃疡区、炎症区结肠组织中的表达较相对正常区明显增加（P〈0．01），较正常对照组也明显增加（P〈0．01）；在蛋白水平上，MMP-1在溃疡区、炎症区的表达较相对正常区明显增加（P〈0．05），较正常对照组也明显增加（P〈0．05）；相对正常区与正常对照组之间的表达无论在mRNA水平还是在蛋白水平差异均无统计学意义；MMP-1的表达在溃疡区、炎症区、相对正常区及正常对照组结肠组织中的阳性率分别为85％、82％、58％、40％；MMP-1与UC病情严重程度具有相关性（r=0．406，P〈0．05）。结论UC病变结肠组织中MMP-1的表达增加，并且与UC的组织损伤程度有关，MMP-1可以作为评价UC病情严重程度的生物学指标；应用外源性基质金属蛋白酶抑制剂治疗可能会成为UC治疗的新途径。

Objective To examine the expression of matrix metalloproteinase - 1 （ MMP - 1 ） in colonic mueosa of patients with ulcerative colitis（UC） , to investigate the role of MMP - 1 in the process of ulceration, and to verify the relationship between MMP- 1 expression and the severity of the disease. Methods Reverse transcription - polymerase chain reaction （ RT - PCR） and immunohistoehemistry were used to study, at both mRNA and protein level, the expression of MMP - 1 in the ulcerated, inflamed and non - inflamed area of colonic mucosa in 40 patients with UC. Biopsy specimens from 10 normal subjects were selected as normal control group. The relationship between MMP - 1 the severity of the disease was analyzed by statistical methods. Results Expression of MMP - 1 mRNA in the ulcerated and ＇inflamed colonic mucosa was significantly higher than that in non - inflamed colonic mucosa （ P 〈 0.01 ） and normal control group（P 〈 0.01 ）. Expression of MMP - 1 at the protein level in the ulcerated and inflamed colonic mucosa was significantly increased when compared with that in non - inflamed colonic mucosa （P 〈 0.05）and in the normal control group（P 〈 0.05 ）. Neither at the mRNA level nor at the protein level the expression of MMP - 1 was statistically different in non - inflamed colonic mucosa and in the normal controls. In the ulcerated, inflamed, non - inflamed colonic mucosa and in the control, the positive rates of MMP - 1 expression were 85% , 82%, 58% , and 40% respectively. Furthermore, the expression of MMP - 1 was well related to the severity of clinical symptoms （ r = 0. 406,P 〈 0.05 ）. Conclusions The increased expression of MMP - 1 is related to colonic tissue damage in UC. MMP - 1 could be used as a biomarker to judge the severity of the disease. Treatment with matrix metalloproteinase inhibitors could be a new way in treating UC.