出生前暴露甲基对硫磷与氯氰菊酯混配农药对大鼠子代神经行为毒性的影响

Neurobehavioral Development in Rat Offspring after Prenatal Exposed to Methylparathion and Cypermethrin

[目的]探讨出生前甲基对硫磷与氯氰菊酯混配染毒,对大鼠子代神经行为发育的影响。[方法]48只Wistar孕鼠随机分为4组,于妊娠第1～15天用农药甲基对硫磷与氯氰菊酯进行连续灌胃染毒,对照组采用溶剂灌胃。甲基对硫磷剂量分别为:0,0.0230,0.0725,0.2300mg/kg体重;氯氰菊酯剂量分别为0,0.8000,2.5265,8.0000mg/kg体重。甲基对硫磷与氯氰菊酯采用等毒性混配,即分别为:0、1/300LD50、1/95LD50、1/30LD50,对349只仔鼠进行早期神经行为发育指标的测试;对64只仔鼠进行Morris水迷宫空间学习和记忆能力的测试。[结果]仔鼠断崖回避达标率在出生的第5、6天,高、中剂量组低于对照组(P<0.05);视觉定位达标率在出生的第16天下午,高、低剂量组低于对照组(P<0.05)。Morris水迷宫实验测试指标,各剂量组与对照组差别无显著性(P>0.05)。[结论]大鼠孕期暴露于甲基对硫磷与氯氰菊酯混配农药,对仔鼠的神经行为发育可产生一定的影响。

[ Objective ] To detect the neurobehavioral effects in rat offspring after prenatal exposure to methylparathion and cypermethrin. [ Methods ] Pregnant Wistar rats were divided randomly into 1 control group and 3 exposure groups. Methylparathion and eypermethrin were force-fed to pregnant rats during 1^st- 15^th days aider conception with the exposure doses of 0, 0.023 0, 0.072 5, 0.230 0 mg/kg BW and 0, 0.800 0, 2.526 5, 8.000 0 mg/kg BW in each group, respectively. Double blind lesl was used in the experiment. The early neurobehavioral development was measured in 349 rat offspring. Meanwhile, spatial learning and memory were also tested with Morris water maze in 64 rat offspring. [ Results ] The rate of reaching the standard in cliff avoidance reflex test was lower in the high and middle exposure groups than in control group at the 5^th and 6^th day after birth（ P 〈 0.05 ）.The visual placing reflex test showed that the performance in the high and low exposure group was lower than the control group. No statistically significant difference in performance of Morris water maze was observed among exposure groups and control group. [ Conclusion ] Prenatal exposure to eypermethrin and methylparathion might have some adverse effeels on the neurobehavioral development in rat offspring.