硫酸铜对小鼠脾细胞DNA损伤研究

Study of the Effects of Copper Sulphate on Splenocyte DNA Strand Breaks in Mice

[目的]观察不同剂量硫酸铜作用不同时间对小鼠脾细胞DNA链断裂损伤的影响。[方法]采用单细胞凝胶电泳法(SCGE)分别测定小鼠灌胃1次染毒硫酸铜50、100、200mg/kg和连续每天1次灌胃硫酸铜12.5、25.0和50.0mg/kg2周及连续灌胃3、6和12mg/kg1个月后小鼠脾细胞DNA链断裂损伤情况。各染毒组均没相应的对照组。[结果]50、100和200mg/kg硫酸铜1次染毒;25.0和50.0mg/kg硫酸铜染毒2周;6和12mg/kg硫酸铜染毒1个月后,脾细胞均出现DNA链断裂损伤的频率以及尾长均明显高于对照组。[结论]硫酸铜可引起脾脏细胞明显的DNA链断裂损伤,此损伤作用存在蓄积效应。

[ Objective ] To observe the effect of copper sulphate on splenocyte DNA strand breaks of mice in different times and different dosages. [ Methods ] Single cell electrophoresis was used to detect the DNA SSB （ single strand breaks ） in splenocyte of mice. The dosages of copper sulphate were different depending on the period of administration, namely 50, 100 and 200 mg/kg for once treatment; 12.5, 25.0 and 50.0 mg/kg for two weeks treatment and 3, 6 and 12 mg/kg for one month treatment. [ Results ] 24 hours after copper sulphate once treatment all three treating dosages induced apparent DNA SSB in splenocyte of mice. After two weeks treatment the DNA SSB in the 25.0 and 50.0 mg/kg copper sulphate treated groups was higher than control group. When copper sulphate was gavaged one month to mice even at the dose of 6 and 12 mg/kg, DNA SSB in splenocyte of mice was apparently increased. [ Conclusion ] Copper sulphate could induce DNA SSB in splenocyte of mice obviously, and there was an accumulative effect.