60岁以下男性心肌梗死患者脂蛋白(a)水平与载脂蛋白(a)表型的关系

The relationship between the concentration of Lp(a) and Apo(a) phenotype in myocardial infarcted men under 60 years old

目的比较60岁以下男性心肌梗死患者和健康对照的脂蛋白[Lp(a)]含量与载脂蛋白(a)[Apo(a)]表型频数分布的关系。方法采用酶法和免疫比浊法测定实验组和对照组的血清脂质,十二烷基硫酸钠-多丙烯酰胺冻胶电泳(SDS-PAGE)法和免疫印迹技术分析Apo(a)表型频数分布。结果两组的Lp(a)含量和Apo(a)表型频数分布差异有统计学意义;心肌梗死组中Apo(a)F、B、S1、S2、S1S2区带出现频率多于对照组,S3、S4和测不到表型者少于对照组;对照组的B、S1、S2表型较其他高相对分子质量Apo(a)表型者具有更高的Lp(a)含量。结论60岁以下男性心肌梗死患者具有更高的Lp(a)含量和更高比例的相对小分子质量Apo(a)表型;可以根据Lp(a)含量和Apo(a)表型预测心血管疾病的发生。

Objective The study was to compare the concentration of Lp（a） and the frequency distribution of the Apo（a） in two groups：myocardial infarcted male group under 60 year old and healthy group.Methods Serum Lp（a） was measured by enzymic method and immunoturbidimetry,and the frequency distribution of Apo（a） was examined by SDS-PAGE and immunoblotting.Results The concentration of Lp（a） and the frequency distribution of two groups were remarkably different.The occurrence frequencys of F,B,S1,S2,S1S2 band in experimental group are higher than that in the controls,and S3,S4 and phenotype-unmeasured are lower than that in the controls. The concentrations of Lp （a） in B, S1, S2 phenotypes of the controls are higher than that in highmolecular weight Apo（a） phenotypes. Conclusion Compared with the controls, the group of myocardial infracted male group under 60 years old display higher Lp（a） concentration and low molecular weight Apo（a） isoforrns. It is useful to predict cardiovascular disease by the concentration of Lp（a） and Apo（a） phenotype.