摘要
采用热水煮提法和乙醇沉淀法从穿山龙中提取出水溶性粗多糖,再经DEAE-Sepharose CL-6B和Sepha-dex G-100柱层析分离纯化得到2个均多糖组分DMA和DMB。利用超氧阴离子实验,羟基阴离子实验和邻苯三酚自氧化实验研究DMA和DMB的抗氧化活性,结果表明DMB有较强的抗氧化活性,应该被作为潜在的抗氧化剂开发利用。
The water-soluble crude polysaccharide, obtained from the Dioscorea nipponica Makino by boiling-water extraction and ethanol precipitation, was fractionated by DEAE-Sepharose CL-6B column chromatography and purified by Sephadex G-100,giving two polysaccharide fractions termed DMA and DMB. On the basis of superoxide radical assay, hydroxyl radical assay and self-oxidation of 1,2,3-phentriol assay, their antioxidant activities were investigated. Among them,DMB had the higher antioxidant activities and should be explored as a novel potential antioxidant agent.
出处
《天然产物研究与开发》
CAS
CSCD
2007年第1期29-34,共6页
Natural Product Research and Development
基金
This work was supported by the Foundation of Fujian Province(Z0516009).
关键词
穿山龙
多糖
纯化
抗氧化活性
Dioscorea nipponica Makino
polysaccharide
purification
antioxidant activities
