鳞盖肉齿菌的抗肿瘤细胞增殖活性及抗肿瘤细胞增殖机制的研究

Anti-tumor Activity and Mechanism of the Compound from Sarcodon scabrosus karst

为了从鳞盖肉齿菌(Sarcodon scabrosuskarst)的二氯甲烷提取物中分离纯化得到Sarcodonin G,对Sarc-odonin G进行抗肿瘤细胞增殖活性及其抗肿瘤细胞增殖机制的研究。本文利用MTT实验法测定Sarcodonin G对HeLa细胞株增殖的抑制率,检测其抗肿瘤细胞增殖的效果;利用流式细胞术检测Sarcodonin G对HeLa细胞的凋亡的影响;利用电镜技术观察Sarcodonin G对HeLa细胞形态学改变。结果发现,Sarcodonin G对体外培养的HeLa细胞的增殖具有明显抑制作用,其IC50为7.19μmol/L,并有较好的剂量依赖关系;流式细胞学检查发现Sarcodonin G处理后的HeLa细胞出现凋亡现象;超微结构发现Sarcodonin G处理后的HeLa细胞的胞核染色质浓缩,边集,核固缩及形成新月体,线粒体肿胀,空泡样变,胞浆出现大量空泡等凋亡的形态学改变。结果表明,鳞盖肉齿菌的纯化物Sarcodonin G能抑制体外培养的HeLa细胞的增殖,并初步推测Sarcodonin G有可能是通过诱导HeLa细胞的凋亡来实现其抗肿瘤增殖作用的。

To study the anti-tumor activity on HeLa cells in vitro and the anti-tumor mechanism of Sarcodonin G,a diterpenoid isolated from the dichloromethane extract of Sarcodon scabrosus karst, the effect of Sarcodonin G on HeLa cancer cell lines was determined by MTT assay in vitro. The influence of Sarcodonin G on the HeLa cell apoptosis was studied with fluorescent staining technique. The morphological changes of HeLa cells were observed by transmission electron microscope. Sarcodonin G could obviously inhibit the proliferation of cultured HeLa cells. The IC50 was 7. 19 μmol/L, and it existed good relationship with the dosage; the apoptosis of HeLa cells induced by Sarcodonin G showed a marked difference in untreated HeLa cells （ P 〈 0.01 ）. The morphological changes of HeLa cells incubated with this drug showed chromatin condensation, marginal convergency and nuclear fragmentation. Sarcodonin G can inhibit the growth of HeLa cells in vitro. It is inferred that the anti-tumor activity of Sarcodonin G was achieved by inducing HeLa cell apoptosis.