湖北贝母与川贝母随机扩增引物DNA的鉴别

Differentiation between Fritillaria Hupehensis and Bulbus Fritillariae Cirrhosae with Random Amplified Polymorphic DNA

目的为鉴定湖北贝母,准确区分湖北贝母和暗紫贝母(正品贝母)寻找一种新的鉴别手段。方法采用随机扩增引物DNA(RAPD)法,筛选适于鉴别湖北贝母、暗紫贝母的随机引物。结果总共筛选了20条随机引物,得到一条引物S1能准确区分湖北贝母、暗紫贝母,并且其特异性非常高。结论可利用随机引物S1通过RAPD进行DNA指纹图谱研究准确区分湖北贝母、暗紫贝母。

Objective To seek after a new method for the identification of Fritillaria hupehensis and accurate distinguishment between Fritillaria hupehensis and Fritillaria unibracteata the certified product, Methods Random primers suitable for the distinguishment between Fritillaria hupehensis and Fritillaria unibracteata were screened out with the adoption of the random amplified polymorphic DNA （ RAPD ） technique, Results 20 random primers were screened out in total and oneof which , the primer S1 ,was shown to accurately distinguish between Fritillaria hupehensis and Fritillaria unibracteata with very high specificity. Conclusion The results showed that RAPD marker technique could effectively distinguish between Fritillaria hupehensis and Fritillaria unibracteata