邻苯二甲醛柱前衍生高效液相色谱法测定马氏珠母贝中牛磺酸含量

Determination of Taurine in Pinctada martensii by High Performance Liquid Chromatographic After Derivatization with Opthaldialdehyde

应用邻苯二甲醛柱前衍生高效液相色谱法,测定了马氏珠母贝中牛磺酸,结果表明:采用邻苯二甲醛为柱前衍生剂,与原料中的牛磺酸发生衍生化反应后,以甲醇-乙酸钠溶液(体积比40︰60)为流动相,使用ODSC18分离柱,FLD检测器(EX=338nm,EM=444nm)等色谱条件下检测,牛磺酸的线性范围为2.0～14.0μg/mL,相关系数R2为0.9984,回收率为97.9%～103.0%,RSD=2.146%(n=6),检出限为0.77μg(S/N=3)。

The separation with quantitative analytical methodof taurine in Pinctada martensii was studied by high performance liquid chromatographic （HPLC） after derivatization with opthaldialdehyde. Results showed that taurine in material was separated and quantified on ODS C18 column by HPLC after derivatization with opthaldialdehyde, using mixture of methanol and sodium acetate （ratio of methanol to sodium acetate 40 ： 60） as mobile phase, and detecting at EX：338 and EM：444 nm by FLD detector, the linear ranger of taurine was 2.0-14.0 μg/mL, correlation coefficient R^2=0.998 4, the recoveries were 97.9% -103.0%, RSD=2.146%（n=6）arld detection limit was 0.77 μg （S/N=3）.