ACE基因多态性及其血浆水平与老年脑卒中的关系

Association of ACE gene polymorphism with serum ACE level and cerebral stroke in the elderly

目的探讨老年脑卒中的发生与血管紧张素转换酶(ACE)基因插入/缺失多态性及其血浆ACE水平的关系。方法应用多聚酶链反应(PCR)方法检测ACE基因多态性,同时用比色法测定血浆ACE水平。对89例老年脑卒中患者(缺血性脑卒中50例,出血性脑卒中39例)与51例老年健康对照者进行比较分析。结果老年脑卒中组D等位基因频率显著高于对照组(0.58/0.36,P<0.05),DD基因型患者血清ACE水平亦显著高于对照组[(42.38±16.61)U/L/(31.28±8.64)U/L,P<0.05]。而出血性脑卒中组与缺血性脑卒中组等位基因及血浆ACE水平无显著差异。结论血浆ACE水平和ACE基因多态性有关,D等位基因是老年脑卒中的基因危险因素。

Objective To investigate the relationship between angiotensin converting enzyme （ACE） gene insertion/deletion （I/ D） polymorphism and serum ACE level in elderly patients with cerebral Stroke. Methods Polymorphism of ACE gene was categorized into three genotypes., genotype DD/ID/II. Polymerase chain reaction （PCR） was used to examine ACE gene polymorphism. Compared the distribution of three ACE genotypes frequencies and D allele frequency in 89 patients with cerebral strokes（39 hemorrhage cerebral strokes, 50 ischemia cerebral strokes） in the elderly and 51 healthy with controls,whose serum ACE levels were measured with colorimetric technique. Results The frequency of D allele was higher in the stroke groups （0.58） than in healthy controls （0. 36） （P〈0.05）. A significant difference in serum ACE level was observed between the strokes and controls[（42.38±16.61） U/L and（31.28±8.64） U/L] respectively（P〈0.05）. There were no significant difference in Serum ACE level with the hemorrhagic cerebral strokes and ischemia cerebral strokes. Conclusion Serum ACE level is associated with ACE gene I/D polymorphism. D allele might be a genetic risk factor for stroke in the elderly.