水稻同核异质不育系及其保持系核基因组的SSR分析

SSR Analysis on Nuclear Genome of Isonuclear Alloplasmic Male Sterile Lines and Their Maintainer Lines in Rice (Oryza sativa)

以4套共15份同核异质水稻雄性不育系和保持系为试验材料,应用随机分布于水稻12条染色体上的42对SSR引物,分析其核基因组的遗传多样性和亲缘关系。在15份材料间共检测到63个等位基因,多态性位点百分率为40.48%,平均每个位点的等位基因数为1.5个,平均基因多样性为0.18。4套同核异质不育系的平均相同位点为54.5个,占总等位基因数的86.51%,差异位点为8.5个,占13.49%。同核异质的不育系与保持系间具有平均77.78%的相同位点和22.22%的差异位点。同质异核不育系中则具有平均53.97%的相同位点和46.03%的差异位点。同时,获得了一些不育系和保持系的指纹图谱。根据不育系和保持系聚类分析图,在遗传距离为0.22处划分为3群,第1群包括华农A和华育A不育系及其保持系共8份材料,第2群包括N9A、N10A和N11A等3份科珍2A材料,第3群包括N12A、N13A、N14A和N12-16B等4份珍汕97A同核异质系。同一套同核异质系基本上都优先聚类在一起,与其选育系谱一致。

Four sets of rice isonuclear alloplasmic lines, including 15 male sterile lines and their maintainer lines were analyzed by using 42 SSR markers, which were randomly selected from 12 chromosomes of rice. A total of 63 alleles were detected in the 15 lines. The frequency of polymorphic loci was 40.48%, the average number of alleles per locus was 1.5, and the average gene diversity was 0.18. Four sets of the isonuclear alloplasmic male sterile lines shared 54.5 identical loci, corresponding to 86.51% of the total alleles; and meanwhile, there were 8.5 different loci between them, it was equal to 13.49%. Average 77.78% identical loci and 22.22% different loci were detected between the isonuclear alloplasmic male sterile lines and their maintainer lines. Similarly, average 53.97% identical loci and 46.03% different loci were existed among the homogenous allonucleus male sterile lines. The SSR fingerprints for some male sterile lines and maintainer lines were established. Based on the cluster analysis, the 15 lines could be divided into three groups at the 0.22 of the genetic distance. The first group consists of eight lines including the male sterile lines and their maintainer lines of Huanong A and Huayu A. The second group consists of three sterile lines of Kezhen 2A, including NgA, N10A and NllA. The third group includes four isonuclear alloplasmic lines of Zhenshan 97A, i. e. , N12A, N13A, N14A and N12-16B. The isonuclear alloplasmic lines of the same sets are mostly clustered in the same group, which is consistent with their pedigrees.