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一种用于水稻点突变检测的PCR引物设计方法 被引量：1

A Primer Design Method for Detection of Point Mutation in Rice
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摘要 介绍了一种用于水稻点突变检测的PCR引物设计方法。利用该方法根据12个候选基因序列设计了12对引物,其中10对引物可以正常工作,能从基因组中扩增出特异性条带,合格率为83%,并且成功地在突变体中检测到点突变。这一方法不仅可以用于水稻,也可以用于其他动植物。同时,对引物设计过程中常见问题进行了讨论并给出了解决办法。 A primer design method was developed for detection of point mutation in rice. Twelve primer pairs were designed for detection of point mutation in 12 candidate genes. A single band was amplified successfully with each of 10 out of 12 primers （83%） from the genome and point mutations were detected in IR64 mutants as well. This method can be used for primer design for animals and other plant species also. In addition, common questions encountered in primer design were discussed and the corresponding measures were put forward.
作者 邱福林 邵国军 Hei LEUNG 吴建利 程式华
机构地区 沈阳农业大学 辽宁省农业科学院稻作研究所 国际水稻研究所昆虫和植物病理系 中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室
出处 《中国水稻科学》 CAS CSCD 北大核心 2007年第2期215-219,共5页 Chinese Journal of Rice Science
基金 辽宁省科技基金资助项目(20032092)
关键词 水稻 定向诱导基因组局部突变 点突变检测 引物设计 聚合酶链式反应 rice targeting induced local lesion in genome（TILLING） point mutation detection primer design polymerase chain reaction
分类号 S511 [农业科学—作物学]
  • 相关文献

参考文献11

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二级参考文献12

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共引文献19

同被引文献23

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二级引证文献2

中国水稻科学
2007年 第2期

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