金地鼠颊囊癌模型PCNA、BrdU及光镜观察结果的相关分析

Correlative analysis with proliferating cell nuclear antigen, bromodeoxyuridine,and microscopic observation on golden hamster carcinogenesis model

癌变的早期诊断对提高疗效具有特殊意义。为了寻求简捷、高效、超前、可靠的诊断方法，实验以DMBA（二甲基苯并直）诱导的金地鼠颊囊致癌模型为对象，以PCNA（增殖细胞核抗原）、BrdV（澳脱氧尿嘧啶）等免疫组化技术为手段，以光镜观察诊断为对照，对三种观察结果进行相关分析，发现三者间有高度显著的相关性，证实了免疫组化方法在癌变诊断中有一定的参考价值。实验还发现PCNA和BrdU检测结果比较敏感、简捷，可靠性较好，因而是很有希望的早期诊断癌变的手段之一。

The early diagnosis of carcinomatous conversion has special significance onincerasing effectiveness. Proliferating cell nuclear antigen (PCNA), bromodeoxyuridine (BR-DU ) (immunohistochemistry ways), and microscopic diagnosis were applied and observed ongolden hamster cheek pouch carcinogenisis model induced by dimethylbenzanthracene (DMBA)in order to seek simple, effective,leading and reliable diagnostic way. The results showed thatthree observable means had siginficant correlativity, and demonstrated that immunohis-tochemistry ways practical value in diagnosis of carcinomatous conversion. This study sug-gests that PCNA and BRDU are more sensitive, simple and reliable, and two hopeful methodsof early diagnosis of carcinomatous conversion.