三种胡子鲶及其杂种F_1的乳酸脱氢酶同功酶的比较研究

COMPARATIVE STUDIES ON LACTATE DEHYDROGENASE ISOZYME IN THREE SPECIES OF CATFISH AND THEIR F_1 HYBRIDS

垂直板聚丙烯酰胺凝胶电泳图谱揭示所有实验鱼的LDH-A_4均表现相同的迁移率,革胡子鲶的LDH-B_4比另两种的迁移较缓慢。胡子鲶和斑点胡子鲶具有五条由A、B座位编码的LDH谱带,且其迁移位置对应相同;革胡子鲶则只有三条LDH酶带。全部实验鱼的肝组织、斑点胡子鲶和革胡子鲶的脑组织存在靠近阴极的酶带,杂种斑点胡子鲶×胡子鲶的LDH与双亲的相问,而杂种胡子鲶×革胡子鲶、斑点胡子鲶×革胡子鲶则显示一些由来自双亲的基因编码的杂合带。利用LDH的表型差异对三种鱼的进化关系、遗传特性和杂交育种问题进行讨论。

Lactate dehydrogenasc isozyme of 3 species of catfish,i, e. Glorias fuscus, C. macrocephalus, C, lazera and their F1 hybrids, C. macrocephalus × C. fuscus, C. fuscus×C. lazera and C. macrocephalus × C. lazera, were studied by polyacrylamidc gel clectrophoresis. These catfish all show the same electrophoretic mobility of LDH-A4 isozyme. The B4 isozyme of C. lazera has a slower mobility than that of other two species. C. fuscus and C. macrocephalus shows 5 bands encoded by A, B loci and C. lazera shows 3. Liver homogenatcs reveal LDH-C isozyme that migrated cathodal to the A4 isozyme. This isozyme: has the same mobility in all samples. In C. macrocephalus and C. lazera, the brain tissue also shows the cathodal isozyme. The F1 hybrids posses some LDH isozymes that encoded by the gene loci from their parents. In C. macrocephalus ×C. fuscus, all five bands arc the same as that in its parents. In other two hybrids, the isozyme shows heterozygosity. According to the isozyme phenotypes we discussed evolution, inheritance and cross breeding problems in these catfish.