原子荧光光谱法测定两种中药中痕量汞

Determination of Trace Mercury in Two Chinese Traditional Medicines by Hydride Generation-Atomic Fluorescence Spectrometry

建立了氢化物发生-原子荧光光谱法测定中药(党参、灵芝)中痕量汞。应用AFS-2202a型双道原子荧光分光光度计和断续流动的氢化物发生技术,采用HNO3-H2O2体系和聚四氟乙烯高压釜消解中药样品,同时,研究了硼氢化钾浓度,光电管负高压,灯电流,原子化器高度,屏蔽气和载气流量等因素对测定结果的影响。在选定实验条件下,汞的检出限为0.021μg/L,桃叶(GBW 08501)验证结果与推荐值一致, 6次测定结果,相对标准偏差为O.34%。该方法简便、快速、灵敏、准确,对于实际中药样品的测定得到了满意的结果。

A high-pressure digestion using HNO3-H2O2 system was applied for the pretreatment of traditional Chinese medicine samples and trace mercury in traditional Chinese medicines was determined by hydride generation-atomic fluorescence spectrometry with the interval flow procedure. The experimental conditions and instrumental operation parameters were optimized. Under the optimum conditions, the detection limit is 0. 021μg/L. Trace mercury in the standard reference substance peach leaf （GBW08501, produced by the environmental science committee of Chinese scientific institute） was also determined with the results in good agreement with certified value. The RSD is less than 0. 34 %. This method is simple, rapid, sensitive, convinent and accurate, and can be successfully used for the determination of trace mercury in traditional Chinese medicines.