吖啶类化合物ICR－191诱发人成纤维细胞hprt基因突变与细胞转化的研究

MORPHOLOGICAL TRANSFORMATION AND MUTATIONS AT THE hprt LOCUSINDUCED BY ACRIDINE MUTAGEN ICR-191 IN HUMAN FIBROBLASTS

用ＩＣＲ－１９１处理人成纤维细胞来诱发转化灶形成和抗６－ＴＧ突变以比较这两个不同的遗传终点效应。结果诱发的转化灶与抗６－ＴＧ突变频率都呈剂量相关的增加．通过利用在相同条件下接触同一诱变剂并经过相同时间的处理后指数增生期培养的同一细胞群，将细胞转化和诱变结合在同一试验中同步进行。这一方法对遗传毒致癌物可同时给出可比较的细胞转化和诱变频率的资料。

To compare the effects at the two different genetic end-points,human fibroblast cell strain MSU-1·1 treated with ICR-191 was examined for induction of focus-formation and mutation to TG resistance in a combined assay,The results indicated that a dose-dependent increase in the frequency of both TG-resistant mutants and formation of foci composed of morphologically altered cells was induced.The difference in frequency between induction of TG resistance and focus formation is about 3-fold at the higher dose level.These data were derived from the same cell population exposed to the mutagen under the same condition and given at the same expression period,which allowed to yield simultaneously information on transformation and mutation frequency with comparability for a potential genotoxic carcinogen.