单步萃取-HPLC法测定人血清中卡马西平浓度

Determination of carbamazepine in human plasma concentration by HPLC with single step

目的：建立单步萃取-HPLC法测定人血清中卡马西平浓度的方法。方法：采用Nova-Pak C18柱（3．9mm×150mm，5μm）色谱柱，流动相为甲醇-2mmol·L^-1磷酸二氢钠溶液（pH3．5）（50：50），流速为0．9mL·min^-1，检测波长为285nm，柱温为30℃。结果：卡马西平在1．2～32mg·L^-1范围内线性关系良好，回归方程：A=5348．61C-1891．2，r=0．9981。方法回收率为99．38％，日内、日间RSD为0．46％～2．02％，最低检测质量浓度为0．3mg·L^-1。结论：本法灵敏、准确、快速、专属性强。

OBJECTIVE To establish an HPLC with single step method for the determination of carbamazepine in human serum. METHODS Mobile phasma was composed of methanol-2 mmol·L^-1 NaH2PO4 solution（50：50, pH 3. 5）with a flow rate of 0. 9 mL·min^-1 ,and the detecting wavelength set at 285 nm. RESULTS The calibration curves were linear within the range of 1.2-32 mg·L^-1 （r = 0. 998 1 ）. The relative average recovery was 99.38%. The within day and between-day precision was 0. 85% - 2. 02% ,0. 46% - 1.42% ,respectively,The detection limit was 0. 3 mg·L^-1. CONCLUSION The proposed method is sensitive, accurate and specific.