摘要
目的:建立肾灵Ⅱ号片中川芎嗪的含量测定方法。方法:采用高效液相色谱法,以Agilent Eclipse XDB-C18为分析柱(46mm×250mm,5μm),流动相:甲醇-1%冰醋酸水溶液(34:66),流速:1mL·min^-1,检测波长:282nm。结果:川芎嗪在0.2~4mg·L^-1范围内与峰面积呈良好的线性关系(r=0.9992)。加样回收率为99.36%,RSD=0.83%。结论:该方法准确、操作简便快速,可以作为肾灵Ⅱ号片的质量控制指标之一。
OBJECTIVE To establish a determination method for ligustrazin in Shenling Ⅱ Tablets. METHODS HPLC was adopted in the study. The liquid chromatography was carried out using Agilent Eclipse XDB-C18 (46 × 250 mm,5μm) ,with detection wavelength at 282 nm. The flow rate of mobile phase [methanolwater(containing 1% glacial acetic acid)(34:66)1 was 1 mL·min^-1. RESULTS The calibration curve was linear within the concentration range of 0. 2-4 ug· ml^-1 , r = 0. 999 2, the average recovery was 99. 36%. RSD = 0. 83%. CONCLUSION The established method is accurate, simple and suitable for quality control of Shenling Ⅱ Tablets.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2007年第3期354-355,共2页
Chinese Journal of Hospital Pharmacy