Hu-TLC-SCID小鼠的建立及其对2型登革病毒的免疫应答

Reconstitution of hu-SCID mice transplanted with human tonsillar lymphocytes immunized with dengue 2-virus in vitro and its immune response

利用２型登革病毒（ＤＶ２）体外免疫人扁桃腺淋巴细胞（Ｈｕ－ＴＬＣ）移植给ＳＣＩＤ小鼠，以建立Ｈｕ－ＴＬＣ－ＳＣＩＤ小鼠。并用不同剂量灭活ＤＶ２加强免疫，观察小鼠对ＤＶ２的免疫应答。结果表明，移植Ｈｕ－ＴＬＣ后第２周起，可在Ｈｕ－ＴＬＣ－ＳＣＩＤ小鼠血清中检出入ＩｇＧ，最高达４１２．８６μｇ／ｍｌ。移植后次日用不同剂量灭活ＤＶ２加强免疫，每２周加强１次，首次加强免疫后第２周，Ｈｕ－ＴＬＣ－ＳＣＩＤ小鼠血清中出现抗ＤＶ２特异性人ＩｇＭ；第４周起有特异性人ＩｇＧ产生，最高达１：６４００。其含量和产生动态与抗原量有关。

Human-TLC-SCID mice were constructed by intraperitoneal (i. p. )transfer of human tonsillar lymphocytes (TLC) immunized with dengue virus-2(DV2) in vitro, then boostered with different doses of DV 2 antigen in vivo. The results showed that human immunoglobulin (Ig ) and speciftic human anti DV 2 antibodies could be detected in sera of the hu-TLC-SCID mice. Two weeks after transplantation of the in vitroimmunized hu-TLC ,the highest human IgG concentration was as high as 412. 8ug/ml . The SCID mice transplanted with 1. 5 x 103 human TLC immunized invitrowere giveni. p. botster injections with 0. 5ml of different concentrations of inactivated DV 2. The first injection was given 1 day after the transplantation of human TLC ,and the injection were repeated with 2-WK intervals. Two and four weeks later . specific human anti-DV 2 IgM or IgG could be detected in these sera and the highest titer reached 1 : 6400. The levels of human IgG and specific human anti-DV 2 antibodies were related with the does of DV 2 antigen .