摘要
在ＰＣＲ／ＳＳＯ分型的基础上，用一对ＰＣＲ引物扩增ＤＰＢ１基因后作ＳＳＣＰ分析，发现该方法能够确定那些用ＰＣＲ／ＳＳＯ方法只检出一个等位基因的样本为纯合基因型或是含一个空白基因的杂合子，９个用ＰＣＲ／ＳＳＯ方法只检出一个ＤＰＢ１等位基因的湖南汉族人样本中，用ＰＣＲ－ＳＳＣＰ方法发现１个样本表现为杂合子。用银染的方法使得ＳＳＣＰ更快速、可靠和没有同位素污染，并为探测新的ＨＬＡ等位基因提供了可能性。
ThehomozygousandheterozygousblankalelesoftheHLA-DPB1locus,whicharediffi-culttobediferentiatedbyPCR/SSOmethod,wereidentifiedbyusingSingle-StrandConformationpoly-morphism(SSCP)analysis.ThegenomicDNAsofnineindividuals,whichweredemonstratedtohaveon-lyoneDPB1alelepreviouslybytheSSOtechnique,wereamplifiedwithDPB1specificprimers.There-sultshowedthatDNAofoneindividualfromthemgave4bandscorrespondingtosingle-strandedDNAs,andalsohadcharacteristicheteroduplexesmigratingjustabovethedouble-strandedDNA,whichwassimilartopositiveheterozygotecontrol.ThefactsuggeststhatSSCPwithsilverstainingisarapid,reliable,andnonradioactivemethod,offeringthepossibilityofdetectingnewHLAalelesandestimatingexactlythealelefrequenciesofdiferentHLAloci.
出处
《中华微生物学和免疫学杂志》
CSCD
北大核心
1996年第5期343-345,共3页
Chinese Journal of Microbiology and Immunology
